Evolution of Complex RNA Polymerases: The Complete Archaeal RNA Polymerase Structure
نویسندگان
چکیده
منابع مشابه
Evolution of Complex RNA Polymerases: The Complete Archaeal RNA Polymerase Structure
The archaeal RNA polymerase (RNAP) shares structural similarities with eukaryotic RNAP II but requires a reduced subset of general transcription factors for promoter-dependent initiation. To deepen our knowledge of cellular transcription, we have determined the structure of the 13-subunit DNA-directed RNAP from Sulfolobus shibatae at 3.35 Å resolution. The structure contains the full complement...
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The recent success in reconstitution of RNAPs (RNA polymerases) from hyperthermophilic archaea from bacterially expressed purified subunits opens the way for detailed structure-function analyses of multisubunit RNAPs. The archaeal enzyme shows close structural similarity to eukaryotic RNAP, particularly to polymerase II, and can therefore be used as model for analyses of the eukaryotic transcri...
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ژورنال
عنوان ژورنال: PLoS Biology
سال: 2009
ISSN: 1545-7885
DOI: 10.1371/journal.pbio.1000102